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KMID : 1146920220520040465
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Journal of Pharmaceutical Investigation
2022 Volume.52 No. 4 p.465 ~ p.476
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Alginate-coated chitosan nanoparticles protect protein drugs from acid degradation in gastric media
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Tran Phuong
Park Jeong-Sook
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Abstract
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Purpose: The aim of this study was to design and evaluate chitosan nanoparticles (CS NPs) coated with alginate which protect protein drugs from acid degradation.K The model protein drug used was bovine serum albumin (BSA).
Methods: BSA-loaded CS NPs (BSA-CS NPs) were prepared using the ionic gelation method with sodium tripolyphosphate and the surface of the BSA-CS NPs were coated with sodium alginate (Alg). The optimized alginate-coated BSA-CS NPs (Alg-BSA-CS NPs) were evaluated for BSA degradation in an acidic medium.
Results: The encapsulation efficiency (EE), particle size, polydispersity index, and zeta potential of the prepared Alg-BSA-CS NPs were 95.2%, 476.4 nm, 0.24, and ? 53.8 mV, respectively. An in vitro release study showed that the initial burst release of BSA from the BSA-CS NPs was higher than that from the Alg-BSA-CS NPs. Cytotoxicity analysis revealed that the Alg-BSA-CS NPs were non-toxic to Caco-2 cells. The in vitro cellular uptake of the Alg-BSA-CS NPs in Caco-2 cells was significantly higher than that of the BSA-CS NPs and free BSA. Sodium dodecyl sulfate?polyacrylamide gel electrophoresis showed that the Alg-BSA-CS NPs protected BSA from degradation in an acidic environment.
Conclusion: Alg-BSA-CS NPs are suitable for the oral delivery of protein drugs by preventing protein degradation in acidic environments.
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KEYWORD
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Protein protection, Acid degradation, Nanoparticles, Chitosan, Alginate, Oral delivery
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